Composite

Part:BBa_K4475007

Designed by: Andrew Taylor   Group: iGEM22_FSU   (2022-09-29)


CTB3 Constitutive Strong Protein Generator Yeast


Construct designed for strong expression of the fungal enzyme CTB3 in saccharomyces cerevisiae, utilizing the Yeast GPD (TDH3) Promoter and CYC100 Terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1944
    Illegal XbaI site found at 781
    Illegal XbaI site found at 1138
    Illegal XbaI site found at 1606
    Illegal XbaI site found at 1924
    Illegal XbaI site found at 2062
    Illegal XbaI site found at 2332
    Illegal XbaI site found at 2785
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1944
    Illegal BglII site found at 1069
    Illegal BglII site found at 1243
    Illegal BglII site found at 1972
    Illegal BglII site found at 3244
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1944
    Illegal XbaI site found at 781
    Illegal XbaI site found at 1138
    Illegal XbaI site found at 1606
    Illegal XbaI site found at 1924
    Illegal XbaI site found at 2062
    Illegal XbaI site found at 2332
    Illegal XbaI site found at 2785
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1944
    Illegal XbaI site found at 781
    Illegal XbaI site found at 1138
    Illegal XbaI site found at 1606
    Illegal XbaI site found at 1924
    Illegal XbaI site found at 2062
    Illegal XbaI site found at 2332
    Illegal XbaI site found at 2785
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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